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Mosaic reconstruction imaged using spinning disk confocal microscopy of a flat mount of retina showing retinal ganglion cells retrogradely labeled via fluorogold injection into the superior colliculus in an astrocyte specific Nf1 heterozygote (Nf1 -/+) conditional knock out mouse. Details may be found in Kim et al., Neuroscience. 2010 Sep 29;170(1):178-88.